Isobel was the daughter of David Hoppringle of Smailholm but her mother is unknown. The date and place of her birth have not been found.
Her husband was David Home. They were married, but the date and place have not been found. Their eight known children were John (?-?), George (?-1513), David (?-?), Alexander (?-?), Robert (?-?), Andrew (?-?), Bartholomew (?-?) and Patrick (?-?).